Marcus Rashford is the sixth player to have withdrawn from Gareth Southgate’s England squad this week.

Here, Press Association Sport takes a look at the six unavailable players for the Three Lions’ Euro 2020 qualification opener against the Czech Republic.

John Stones (Manchester City)

John Stones has failed to shake off a groin injury
John Stones failed to shake off a groin injury (Richard Sellers/PA)

Despite being on the bench against Schalke in the Champions League last midweek, manager Pep Guardiola made it clear that the centre-back is troubled by a groin injury and as a result omitted him from his FA Cup squad.

Fabian Delph (Manchester City)

Fabian Delph, left, played just under an hour against Swansea last weekend
Fabian Delph, left, played just under an hour against Swansea last weekend (Nick Potts/PA)

Delph was replaced less than an hour into Manchester City’s FA Cup quarter-final win at Swansea last weekend and withdrew from the squad with an unspecified issue.

Ruben Loftus-Cheek (Chelsea)

Ruben Loftus-Cheek was a second-half substitute against Everton on Sunday, but pulled out of the England Squad on Monday
Ruben Loftus-Cheek was a second-half substitute against Everton on Sunday, but pulled out of the England Squad on Monday (Adam Davy/PA)

The midfielder came on as a second-half substitute as Chelsea lost to Everton on Sunday but withdrew the following day through an undisclosed issue.

Luke Shaw (Manchester United)

Luke Shaw also pulled out on Monday with an unspecified problem
Luke Shaw also pulled out on Monday with an unspecified problem (John Walton/PA)

Played the full 90 minutes as Manchester United bowed out of the FA Cup at Wolves, but a reoccurring complaint saw the left-back pull out on Monday.

Trent Alexander-Arnold (Liverpool)

A back injury has ruled out full-back Trent Alexander-Arnold
A back injury has ruled out defender Trent Alexander-Arnold (Adam Davy/PA)

Came to St George’s Park out of respect to Southgate and in a bid to overcome a back injury picked up when Liverpool played 10 days earlier. Made progress but returned to his club on Wednesday.

Marcus Rashford (Manchester United)

Marcus Rashford scored a late consolation in the FA Cup quarter final match against Wolves, but could not shake off an ankle injury
Marcus Rashford scored a late consolation in the FA Cup quarter final match against Wolves, but the Manchester United striker could not shake off an ankle injury (Chris Radburn/PA)

Played the entirety of United’s FA Cup loss at Molineux, scoring at the end, but has been unable to shake an ankle injury during individual sessions. Returned to United on Thursday.